POTENSI PENGINDUKSI APOPTOSIS SENYAWA NIKOTINIL DIBUTIL GLUTAMAT ESTER TERHADAP SEL KANKER T47D

Chandra Risdian, Tjandrawati Tjandrawati, Muhamad Hanafi, Yulia Anita, AMJ Putra

Abstract


Nicotinil Dibuthyl Glutamate Ester (NDBGE) is a synthetic compound that is an analog of antibiotic UK3A from mycelium of Streptomyces sp.517-02. This compound was estimated to have anticancer activity similar to UK-3A. The aim of this research was to know the NDBGEs apoptotic inducing potency to human breast cancer T47D in vitro. DMEM + 1% PS + 10% FBS was added to human breast cancer T47D cells until cell concentration reached 1-5x/ 05eells/ml. NDBGE then was diluted by DMSO until 100ppm concentration. Propidium iodide with 50pg/m1 concentration was added to the T47D cells. The analysis was conducted by flow cytometry method. The result was that the apoptosis percentage of the cells was 12,35%.

 

 


Keywords


Nicotinil Dibuthyl Glutamate Ester, Human breast cancer T47D, Apoptosis, Flow cytometry

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DOI: http://dx.doi.org/10.14203/widyariset.12.1.2009.149-154

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