POTENSI ANTIOKSIDAN DAN AKTIVITAS ANTIPROLIFERASI EKSTRAK KUNYIT PUTIH (Curcuma zedoaria Rosc.) PADA SEL HELA

Saefudin Saefudin, Fauzia Syarif, Chairul Chairul

Abstract


The aim of this study is to determine the antioxidant potential and anti-proliferative activity of Curcuma zedoaria Rosc extract on Hela cells. Two types of materials, such as water and ethanol extracts, were tested at different dose levels (1, 5, and 10%). The potential of antioxidant extract was tested by observing the peroxide value (POV) and prophylactic properties of superoxide (SO). The effects of Cytotoxic extracts were tested by observing the anti-proliferative properties on Hela cell morphology. Results showed that both water extract and ethanol extract contain antioxidants with higher value of superoxide and peroxide than a-tocopherol. Antioxidant potential of Curcuma zedoaria extract is evidential at its high concentration of 10%, but is less significant at lower concentration of 1 and 5%, when compared to the potential of positive control (a-tocopherol) and negative control (non-extract mixture). Antiproliferative activity of Curcuma zedoaria extracts at low concentration (1 and 5%) is not effective. At high concentration of 10%, anti-proliferative activity is effective. Cytotoxic effect of water extract of the rhizome of Curcuma zedoaria at the concentration of 10% changes the Hela cell morphology, but not cell wall rupture. High concentration of ethanol extract causes Hela cells to change its shape, rupture the cell walls, and fragment the cells.

Keywords


Antioxidant; Cytotoxic effect; Antiproliferative activity; Extracts; Curcuma zedoaria

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DOI: http://dx.doi.org/10.14203/widyariset.17.3.2014.381-389

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