CHARACTERIZATION OF SOME MORPHOLOGY, ANATOMY AND  PHYSIOLOGY OF THE WHEAT MUTANT (Triticum aestivum L.) DEWATA  AND SELAYAR IN TROPICAL LOWLAND.

Laela Sari; Agus Purwito; Didy Sopandie

doi:10.14203/widyariset.1.1.2015.%p

CHARACTERIZATION OF SOME MORPHOLOGY, ANATOMY AND PHYSIOLOGY OF THE WHEAT MUTANT (Triticum aestivum L.) DEWATA AND SELAYAR IN TROPICAL LOWLAND.

Laela Sari, Agus Purwito, Didy Sopandie

Abstract

Wheat mutant characterization is a part of the breeding programs to investigate the diversity that can influence in production increased. The aim of this research was to obtain data characterization morphology, anatomy and physiology that could be used as selection criteria and obtain an adaptive wheat mutant in the tropical lowland area. The research was conducted in Seameo-Biotrop Experimental Garden, located on ±250 m above the sea level, from April until November 2013. There were 18 Dewata wheat mutants and two Selayar wheat mutants, which is an M3 derivative resulted from EMS treatment, were used. LC50 Dewata got the 0.3% EMS treatment for 30 minutes, while LC50 Selayar got the 0.1% EMS treatment for 60 minutes. The data analysis used Augmented Designs Method. The results showed that the mutant Dewata that was significantly affected the morphology was indicated by four characters i.e. cooking time (two mutants), grain weight per genotype (seven mutants), leaf area (five mutants), and green leaves (one mutant). There are two characters in mutant Selayar that bring significant influence on morphological factors, those are seed weight per genotype (one mutant) and leaf area (one mutant), while the cooking time and green leaves have no real effect. Anatomical character appearance on leaf thickness and the size of stomata showed different levels of tolerance between Dewata plant mutant (DW-0,3.30-2-13-3), Selayar mutant (SL-0,1.60-2-14-2), and its both controls. As for the physiological character, there were significant differences in the amount of proline and glucose levels. Proline level in Dewata control was 79.29 µg/gBB, while in DW mutant -0,3.30-2-13-3 was 332.37 µg/gBB. Proline levels in Selayar control was 201.53 µg/gBB, while in mutant SL mutant-0,1.60-2-14-2 was 335.79µg/gBB. Likewise, glucose level in Dewata control was 14.32mg/gBB, while in DW mutant 0,3.30-1-15-1 was 29.06 mg/gBB, Selayar controls (5.87 mg /GBB) with SL mutant-0,1.60-2-14-2 (17.68 mg /gBB).

Keywords

Some characterization; Wheat (Triticum aestivum L.); Dewata and Selayar mutant; EMS; Tropical lowland

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References

Warren, R.N., Amell, N., Nichols, R., Levy, P., dan Price, J., 2006.Understanding the Regional Impact of Climate Change. Research Report Prepared for the Stern Review, Tyndall Center Working Paper 90, Norwich. (www.tyndall.ac.uk/publications/working.paper/twp90.pdf.)

Danakusuma, T., 1985. Hasil penelitian Gandum dan prospek pengembangannya. Didalam:Subandi et al. (Eds). Risalah Rapat Teknis Hasil Penelitian Jagung. Sorgum dan Gandum Puslitbangtan, Bogor. hlm 189–202.

Reynolds, M.P., 2002. Physiological approaches to wheat breeding. Dalam: Curtis, B.C., Rajaram, S., dan Macpherson, H.G. (Ed.) Bread wheat improvement and production. Roma:FAO. 567 hlm.

P3TP., 2008. Prospek dan arah pengembangan agribisnis gandum. Badan Penelitian dan Pengembangan Pertanian.

Gayatri, B., Subandi, Sutjihno, Kusuma R., 1989. Risalah Seminar Hasil Penelitian Tanaman Pangan Balittan Bogor.1: 108–114.

Dasmal, A., Kaher, M., dan Yusuf., 1994. Penampilan galur-galur terigu pada dua waktu tanam. Pemberitaan Penelitian Sukarami.23: 8–11.

Nur, A., 2013. Adaptasi tanaman gandum (Triticum aestivum L.) toleran suhu tinggi dan peningkatan keragaman genetik melalui induksi mutasi dengan menggunakan irradiasi sinar gamma. Desertasi, Sekolah Pascasarjana. Institut Pertanian Bogor.

Watanabe, N., Fujii, C., Shiroto, M., dan Furuta, Y., 1991. Changes in chlorophyll, thylakoid protein and photosynthetic adaptation to sun and shade environment in diploid and tetraploid Oryza punctuateKotschy and diploid Oryza eichingeri peter. Plant Physiol. Biochem. Paris. 31(4):469–474.

Hoogenboom, G., Peterson, C.M., dan Huck, M.G., 1987. Shoot growth rate of soybean as affected by drought stress. Agron. J.79(4): 598–607.

Sari, L., Purwito, A., Soepandi, D., Purnamaningsih, R., dan Sudarmonowati, E., 2015. The morphology, anatomy and physiology characterization of mutant wheat (Triticum aestivumL) “Alibey” in tropical lowland area. J. Bio. & Env. Sci. 6(1): 395–403.

Maestri, E., Klueva, N., Perrotta, C., Gulli, M., Nguyen, H.T., dan Marmiroli, N., 2002. Molecular genetics of heat tolerance and heatshock proteins in cereals. Plant Mol. Biol. 48: 667–681.

Ferris, R., Ellis, R.H., Wheeler, T.R., dan Hadley, P., 1999. Effect of high temperature stress at anthesis on grain yield and biomass of field grown crops of wheat. Plant Cell Environ. 34: 67–78.

Sakin, M.A., 2002. The use of induced micro mutation for quantitative characters after EMS and Gamma ray treatments in Durum wheat breeding. Pakistan Journal of App.Science 2(12): 1102–1107.

Luan, Y.S., Juan, Z., Gao, R.X., dan An, L.J., 2007. Mutation induced by ethylmethanesulphonate (EMS) in vitro screening for salt tolerance and

plant regeneration of sweet potato (Ipomea batatasL.). Plant Cell Tissue Organ Cult.88(1): 77–81.

Van Harten, A.M., 1998. Mutation Breeding: Theory and Practical Applications. New York. Cambridge Univ Pr. 353 hlm.

Viswanathan, P., dan Reddy, V.R.K., 1996. Genetics of early flowering mutants intriticale. Acta Agronomica Hungaria46(4): 389–391.

Petersen, R.G., 1994. Agricultural Field Experiments, Design and Analysis. New York. USA: Marcel Dekker Inc.

Capellades, M., Fontarnau, R., Carulla C., dan Debergh P., 1990.Environment influences anatomy of stomata and epidermal cells in tissue cultured Rosa multiflora. J.AMER.Soc.HORT.SCI. 115(1): 141–145.

Bates, L.S., Waldren, R.P., dan Teare., 1973. Rapid determination of free proline for water stress studies. Plan Soils39: 205–207.

World Development Report., 2008. Global Warming. Adaptasi Pertanian dalam Pemanasan Global.(http://www.michellehenry.fr/warming40.gif.)

Sari L, Purwito, A., Soepandi, D., Purnamaningsih, R., dan Sudarmonowati,E., 2014. Wheat (Triticum aestivumL.) mutants throught in vitro selection tolerant on lowland tropic. Int. J. Agri. Res. 5(5):189–199.

Handoko. 2007. Penelitian dan Pengembangan Gandum di Indonesia.Seameo-Biotrop. Bogor. Indonesia. 118 hlm.

[BMKG] Badan Meteorologi, Klimatologi dan Geofisika., 2013. Data iklim Bogor Tahun 2013. Bogor (ID): Stasiun Klimatologi Darmaga Bogor.

Van Ginkel, M., dan Villareal, R.L., 1996. Triticum L., In Grubben GJH,Partohardjono S.(Eds.). Plant resourse of South-East Asia (PROSEA), No.10. Cereals. Leiden, Netherland: Backhuys Publisers. p.137-143.

Dolferus, R., Xuemei, J.I., dan Richard, A.R., 2011. Abiotic stress and control of grain number in cereal. Plant Sci.181:331–341.

Savicka, M., dan Skute, N., 2012. Some morphological, physiological and biochemical characteristics of wheat seedling Titicum aestivumL. organs after high-temperature treatment. Ekologija58(1):9–12.

Ivory, D.A., 1989. Site characteristik in De lacy,LH.(Ed). Analysis of data from agriculture adaptation experiments. Australia Cooperation with the Thai/World Bank National Agricultural Research Project (ACNARP) Training Course. Suphanburi and Chiang Mai Thailand: 15-17 January 1989. 17–24hlm.

Thuzar, M., Puteh, S.B., Abdullah, N.A.P., Mohd Lassim, M.B., dan Jusoff, K., 2010. The effect temperature stress on the quality and yield of soya bean (Glycine max.L.Merril.). J. Of Agriculture Science. 2(1):172–179.

Taiz, L, dan Zeiger, E., 2002. Plant Physiology. Third edition. Sinauer Associates Inc. Publishers, Massachusetts.

Sopandie, D., 2014. Fisiologi Adaptasi Tanaman terhadap Cekaman Abiotik pada Agroekosistem Tropika. Bogor: IPB Press. P.228.

Patil, R.P., 2008. Physiological approaches for drought tolerance in sugarcane. Disertasion. University of Agricultural Sciences, Dharwad.

Bandurska, H., 2000. Does proline accumulated in leaves of water devicit stressed barley plant confine cell membrane injury? I. Free proline accumulation and membran injury index in drought and osmotically stressed plant. Acta Physiologiae Plantarum22(4):409–415.

DOI: http://dx.doi.org/10.14203/widyariset.1.1.2015.%25p

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