DIVERSITY OF ENDOPHYTIC BACTERIA ASSOCIATED WITH (Curcuma heyneana) AND THEIR POTENCY FOR NITROGEN FIXATION

Tri Ratna Sulistiyani, Puspita Lisdiyanti

Abstract


Endophytic bacteria shows a high biodiversity and some of the species plays important biological roles in agriculture. The aim of this study was to investigate the endophytic bacteria diversity associated with temu giring (Curcuma heyneana) and to evaluate its nitrogen-fixation activity. Temu giring was collected from Bogor Botanic Garden. The isolation of  endophytic bacteria was carried out using two methods (spread plate and plant piece methods) and four different medias (Nutrient Agar (NA), NA contained temu giring extract (NAH), Water Yeast Extract Agar (WYEA), and WYEA contained  temu  giring extract (WYEAH)). The identification of selected isolates were conducted based on 16S rDNA. The ability of selected isolates to fix the nitrogen on Jensen’s media is then being tested. The results revealed that the suitable method and media of endophytic bacteria isolation were spread plate method and NA. Based  on  the  morphological characteristics differentiation, 30 isolates were obtained from rhizome (27%), stem (50%), and leaves (23%). The sequencing result of 16S rDNA showed that community of endophytic bacteria was divided into six clusters, those are Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Flavobacteriia, Actinobacteria, and Firmicutes,which represented 17 genus consisted of Microbacterium, Leclercia, Brevundimonas, Chromobacterium, Enterobacter, Acinetobacter, Novosphingobium, Chryseobacterium, Curtobacterium, Agrobacterium, Sphingomonas, Herbaspirillium, Bacillus, Variovorax, Mycobacterium,  Starkeya, and Rhizobium. A total of eleven isolates could grow in the N free medium. The presence of endophytic bacteria those were able to fix  nitrogen are expected to be applied in agricultural sector as a biological fertilizer.

Keywords


Curcuma heyneana; Endophytic bacteria; Population diversity; Nitrogen-fixing activity

Full Text:

References


Ahmad, Farah, Iqbal Ahmad, and M.S. Khan. 2008. “Screening of Free-Living Rhizospheric Bacteria for Their Multiple Plant Growth Promoting Activities.” Microbiological Research 163 (2): 173–81. doi:10.1016/j.micres.2006.04.001.

Chebotar, V. K., N. V. Malfanova, a. V. Shcherbakov, G. a. Ahtemova, a.

Y. Borisov, B. Lugtenberg, and I. a. Tikhonovich. 2015. “Endophytic Bacteria in Microbial Preparations That Improve Plant Development (Review).” Applied Biochemistry and Microbiology51 (3): 271–77. doi:10.1134/S0003683815030059.

Chen, T., Z. Chen, G. H. Ma, B. H. Du, B. Shen, Y. Q. Ding, and K. Xu. 2014.“Diversity and Potential Application of Endophytic Bacteria in Ginger.” Genetics and Molecular Research

(3): 4918–31. doi:10.4238/2014. July.4.6.

Cho, Woong, Joo-Won Nam, Hyun-Jun Kang, Tri Windono, Eun-Kyoung Seo, and Kyung-Tae Lee. 2009. “Zedoarondiol Isolated from the Rhizoma of Curcuma heyneanaIs Involved in the Inhibition of iNOS, COX-2 and pro-Inflammatory Cytokines via the Downregulation of NF-kappaB Pathway in LPSStimulated Murine Macrophages.” International Immunopharmacology 9 (9): 1049–57. doi:10.1016/j.intimp.2009.04.012.

Dalal, Jitendra, and Nikhilesh Kulkarni. 2013. “Population Dynamics and

Diversity of Endophytic Bacteria Associated with Soybean (Glycine Max (L) Merril).” British Microbiology Research Journal 3 (1): 96–105.

Diastuti, Hartiwi, Yana Maolana Syah, Lia Dewi Juliawaty, and Marlia Singgih. 2014. “Antibacterial Activity of Germacrane Type Sesquiterpenes

from Curcuma heyneanaRhizomes.” Indo J. Chem14 (1): 32–36.

Erko, Stackebrand, and Jonas Ebers. 2006. “Taxonomic Parameters Revisited: Tarnished Gold Standards.” Microbiology Today33: 152–55.http://scholar.google.com/scholar?hl=en&btnG=Search q=intitle:Taxonomic+parameters+revisited:+tarnished+gold+standards#0.

Franche, Claudine, Kristina Lindström, and Claudine Elmerich. 2009. “Nitrogen Fixing Bacteria Associated with Leguminous and Non-Leguminous Plants.” Plant and Soil321 (1-2): 35–59. doi:10.1007/s11104-008-9833-8.

Hasimun, Patonah, I K Adnyana, Rizka Valentina, and Euis Lisnasari.

“Potential Alpha-Glucosidase Inhibitor From Selected Zingiberaceae

Family” 9 (1).

Kumar, Maheep. 2014. “Bacteria Involving in Nitrogen Fixation and Their

Evolutionary Correlation.” International Journal of Current Microbiology

and Applied Sciences3 (3): 824–30.

M, joseph B and priya. n.d. “(Print) Bioactive Compounds from Endophytes.pdf.”

Mano, Hironobu, and Hisao Morisaki. 2008. “Endophytic Bacteria in the

Rice Plant.” Microbes and Environments 23 (2): 109–17. doi:10.1264/jsme2.23.109.

McGinnis, Scott, and Thomas L. Madden. 2004. “BLAST: At the Core of a Powerful and Diverse Set of Sequence Analysis Tools.” Nucleic Acids Research 32 (WEB SERVER ISS.): 20–25. doi:10.1093/nar/gkh435.

Miller, Kristin I., Chen Qing, Daniel Man Yuen Sze, Basil D. Roufogalis, and

Brett A. Neilan. 2012. “Culturable Endophytes of Medicinal Plants and the

Genetic Basis for Their Bioactivity.” Microbial Ecology64 (2): 431–49. doi:10.1007/s00248-012-0044-8.

Ogbo, Frank, and Julius Okonkwo. 2012. “Some Characteristics of a Plant Growth Promoting Enterobacter Sp. Isolated from the Roots of Maize”

(September): 368–74.

Okumura, Ricardo Shigueru, Daiane de Cinque Mariano, Rivanildo Dallacort, Amanda Nogueira de Albuquerque, Allan Klynger da Silva Lobato, Elaine Maria Silva Guedes, Cândido Ferreira de Oliveira Neto, Heráclito Eugênio da Conceição, and Gustavo Antonio Ruffeil Alves. 2013. “Azospirillum: A New and Efficient Alternative to Biological Nitrogen Fixation in Grasses.” Journal of Food, Agriculture and Environment 11 (1): 1142–46.

Packeiser, Heiko, Chanyuen Lim, Balaji Balagurunathan, Jinchuan Wu, and

Hua Zhao. 2013. “An Extremely Simple and Effective Colony PCR

Procedure for Bacteria, Yeasts, and Microalgae.” Applied Biochemistry and Biotechnology169 (2): 695–700. doi:10.1007/s12010-012-0043-8.

Powers, E. M. 1995. “Efficacy of the Ryu Nonstaining KOH Technique for Rapidly Determining Gram Reactions of Food-Borne and Waterborne Bacteria and Yeasts.” Applied and Environmental Microbiology 61 (10): 3756–58.

Ratna Sulistiyani, Tri, Puspita Lisdiyanti, and Yulin Lestari. 2014. “Population and Diversity of Endophytic Bacteria Associated with Medicinal Plant Curcuma zedoaria.” Microbiology Indonesia8 (2): 65–72. doi:10.5454/

mi.8.2.4.

Reis, V. M., P. Estrada-de los Santos, S. Tenorio-Salgado, J. Vogel, M. Stoffels, S. Guyon, P. Mavingui, et al. 2004. “Burkholderia Tropica sp. Nov., a Novel Nitrogen-Fixing, Plant-Associated Bacterium.” International Journal of Systematic and Evolutionary Microbiology54 (6): 2155–62. doi:10.1099/ijs.0.02879-0.

Riyanto S. 26 April 2010. Yuk, Bikin Cantik Kulit. www.htttp://agrinaonline.com/show_article.php?rid=12&aid=2417.didownload pada 22 Juni 2016.

Rosenblueth, Mónica, and Esperanza Martínez-Romero. 2006. “Bacterial Endophytes and Their Interactions with Hosts.” Molecular PlantMicrobe Interactions : MPMI19 (8): 827–37. doi:10.1094/MPMI-19-0827.

Ruby, E Jalgaonwala. 2011. “A Review: Bacterial Endophytes and Their Bioprospecting.” Journal of Pharmacy Research4 (3): 795–99.http://www.doaj.org/doaj?func=abstract&id=804173.

Saitou, N, and M Nei. 1987. “The NeighbourJoining Method: A New Method for Reconstructing Phylogenetic Trees.” Mol Biol Evo4: 406–25.

Santi, Carole, Didier Bogusz, and Claudine Franche. 2013. “Biological Nitrogen Fixation in Non-Legume Plants.” Annals of Botany111 (5): 743–67.

doi:10.1093/aob/mct048.

Strobel, Gary, and Bryn Daisy. 2003. “Bioprospecting for Microbial Endophytes and Their Natural Products.” Microbiology and Molecular Biology Reviews: MMBR 67 (4): 491–502. doi:10.1128/MMBR.67.4.491-502.2003.

Tamura, Koichiro, Glen Stecher, Daniel Peterson, Alan Filipski, and Sudhir

Kumar. 2013. “MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0.” Molecular Biology and Evolution30 (12): 2725–29. doi:10.1093/molbev/mst197.

Tan, R X, and W X Zou. 2001. “Endophytes: A Rich Source of Functional

Metabolites.” Natural Product Reports18 (4): 448–59. doi:10.1039/b100918o.

Vendan, Regupathy Thamizh, Young Joon Yu, Sun Hee Lee, and Young Ha Rhee. 2010. “Diversity of Endophytic Bacteria in Ginseng and Their Potential for Plant Growth Promotion.” Journal of Microbiology (Seoul, Korea) 48 (5): 559–65. doi:10.1007/s12275-010-0082-1.

Wei, Chun Yan, Li Lin, Li Jing Luo, Yong Xiu Xing, Chun Jin Hu, Li Tao Yang, Yang Rui Li, and Qianli An. 2014. “Endophytic Nitrogen-Fixing Klebsiella Variicola Strain DX120E Promotes Sugarcane Growth.” Biology and Fertility of Soils50 (4): 657–66. doi:10.1007/s00374-013-0878-3.

Youseif, Sameh H., Fayrouz H. Abd El-Megeed, Amr Ageez, Zeinat K. Mohamed, Abdelaal Shamseldin, and Saleh A. Saleh. 2014. “Phenotypic Characteristics and Genetic Diversity of Rhizobia Nodulating Soybean in Egyptian Soils.” European Journal of Soil Biology 60: 34–43. doi:10.1016/j.ejsobi.2013.10.008.




DOI: http://dx.doi.org/10.14203/widyariset.2.2.2016.106-117

Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 Widyariset

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Indexed by :